English Version
所况简介
所长致辞
现任领导
历任领导
组织机构
园区风貌
学术委员会
学位委员会
  当前位置 >> Development of real-
 

Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

 

Yang Y, Qin X, Zhang X, Zhao Z, Zhang W, Zhu X, Cong G, Li Y, Zhang Z

 

Virol J

2017 Jul 17;14(1):131

Abstract:

BACKGROUND:

Goatpox virus (GTPV) and sheeppox virus (SPPV), which belong to the Capripoxvirus (CaPV), are economically important pathogens of small ruminants. Therefore, a sensitive, specific and rapid diagnostic assay for detection of GTPV and SPPV is necessary to accurately and promptly control these diseases.

METHODS:

Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively.

RESULTS:

The sensitivity of both CaPV real-time RPA assay and CaPV RPA LFD assay were 3 × 102 copies per reaction within 20 min at 38 °C. Both assays were highly specific for CaPV, with no cross-reactions with peste des petits ruminants virus, foot-and-mouth disease virus and Orf virus. The evaluation of the performance of these two assays with clinical sample (n = 107) showed that the CaPV real-time RPA assay and CaPV RPA LFD assay were able to specially detect SPPV or GTPV present in samples of ovine in liver, lung, kidney, spleen, skin and blood.

CONCLUSIONS:

This study provided a highly time-efficient and simple alternative for rapid detection of GTPV and SPPV.

 

 
访问总人数:11892272今日IP访问量:1今日浏览量:395统计天数:2794平均日访问量:4256
设为首页 加入收藏 联系我们 英文版网站
中国农业科学院兰州兽医研究所 网站首页网址:www.chvst.com 网站负责人姓名:李潇萍
陇ICP备13000775号-1 网站维护:本站由兰州中林智能科技有限公司维护